TUNEL Cancer Research Results

TUNEL, Terminal deoxynucleotidyl transferase dUTP nick end labelin: Click to Expand ⟱
Source:
Type:
The TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) assay is widely used to detect DNA fragmentation associated with apoptosis. Rather than being a gene or protein whose expression is regulated, TUNEL is a methodological marker that reveals the extent of apoptosis within tumor tissues.

TUNEL positivity is highly influenced by treatment. High apoptosis after chemotherapy or radiotherapy is generally viewed as a positive indicator of treatment effectiveness, whereas high basal apoptosis in untreated tumors can sometimes be associated with high tumor turnover and aggressiveness.
Comprehensive analysis (often combining TUNEL with proliferation markers such as Ki-67) is needed for accurate prognostication.
Scientific Papers found: Click to Expand⟱
4455- DFE,    Ajwa Date (Phoenix dactylifera L.) Extract Inhibits Human Breast Adenocarcinoma (MCF7) Cells In Vitro by Inducing Apoptosis and Cell Cycle Arrest
- in-vitro, BC, MCF-7 - in-vitro, Nor, 3T3
TumCCA↑, demonstrated cell cycle arrest at 'S' phase; increased p53, Bax protein expression; caspase 3activation and decreased the mitochondrial membrane potential (MMP)
P53↑,
BAX↑,
Casp3↑,
MMP↓,
Fas↑, Quantitative real time PCR (qRT-PCR) analysis showed up-regulation of p53, Bax, Fas, and FasL and down-regulation of Bcl-2.
FasL↑,
Bcl-2↓,
Apoptosis↑, MEAD inhibited MCF7 cells in vitro by the inducing cell cycle arrest and apoptosis
TumCP↓, MEAD inhibited MCF7 proliferation
TUNEL↑, MEAD for 48 h showed dose dependent increase in the numbers of TUNEL positive cells compared to the untreated control
eff↑, Given the folklore claims of cancer inhibitory properties of Ajwa date extract and our results on MCF7 cells, the date fruits could be added daily as a nutritional supplement for synergistic chemopreventive effects against breast cancer and other mal
selectivity↑, IC50, 50 mg/ml vs 18.2 mg/ml

1434- SFN,  GEM,    Sulforaphane Potentiates Gemcitabine-Mediated Anti-Cancer Effects against Intrahepatic Cholangiocarcinoma by Inhibiting HDAC Activity
- in-vitro, CCA, HuCCT1 - in-vitro, CCA, HuH28 - in-vivo, NA, NA
HDAC↓,
ac‑H3↑,
ChemoSen↑, SFN synergistically augmented the GEM-mediated attenuation of cell viability and proliferation
tumCV↓,
TumCP↓,
TumCCA↑, G2/M cell cycle arrest
Apoptosis↑,
cl‑Casp3↑,
TumCI↓,
VEGF↓, VEGFA
VEGFR2↓,
Hif1a↓,
eNOS↓,
EMT?, SFN effectively inhibited the GEM-mediated induction of epithelial–mesenchymal transition (EMT)
TumCG↓,
Ki-67↓,
TUNEL↑, increased TUNEL+ apoptotic cells
P21↑,
p‑Chk2↑,
CDC25↓, decreased p-Cdc25C
BAX↑,
*ROS↓, SFN is also known to exert anti-oxidative effects via Nrf2 activation. in vivo study, optimization is performed by evaluating the anti-oxidative property of SFN in the liver.
NQO1?, identified 50 mg/kg/day as the minimal dose that significantly induced these anti-oxidative genes


Showing Research Papers: 1 to 2 of 2

* indicates research on normal cells as opposed to diseased cells
Total Research Paper Matches: 2

Pathway results for Effect on Cancer / Diseased Cells:


Redox & Oxidative Stress ⓘ

NQO1?, 1,  

Mitochondria & Bioenergetics ⓘ

CDC25↓, 1,   MMP↓, 1,  

Cell Death ⓘ

Apoptosis↑, 2,   BAX↑, 2,   Bcl-2↓, 1,   Casp3↑, 1,   cl‑Casp3↑, 1,   p‑Chk2↑, 1,   Fas↑, 1,   FasL↑, 1,   TUNEL↑, 2,  

Transcription & Epigenetics ⓘ

ac‑H3↑, 1,   tumCV↓, 1,  

DNA Damage & Repair ⓘ

P53↑, 1,  

Cell Cycle & Senescence ⓘ

P21↑, 1,   TumCCA↑, 2,  

Proliferation, Differentiation & Cell State ⓘ

EMT?, 1,   HDAC↓, 1,   TumCG↓, 1,  

Migration ⓘ

Ki-67↓, 1,   TumCI↓, 1,   TumCP↓, 2,  

Angiogenesis & Vasculature ⓘ

eNOS↓, 1,   Hif1a↓, 1,   VEGF↓, 1,   VEGFR2↓, 1,  

Drug Metabolism & Resistance ⓘ

ChemoSen↑, 1,   eff↑, 1,   selectivity↑, 1,  

Clinical Biomarkers ⓘ

Ki-67↓, 1,  
Total Targets: 31

Pathway results for Effect on Normal Cells:


Redox & Oxidative Stress ⓘ

ROS↓, 1,  
Total Targets: 1

Scientific Paper Hit Count for: TUNEL, Terminal deoxynucleotidyl transferase dUTP nick end labelin
Query results interpretion may depend on "conditions" listed in the research papers.
Such Conditions may include : 
  -low or high Dose
  -format for product, such as nano of lipid formations
  -different cell line effects
  -synergies with other products 
  -if effect was for normal or cancerous cells

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