Quercetin / Hif1a Cancer Research Results

QC, Quercetin: Click to Expand ⟱
Features:
Plant pigment (flavonoid) found in red wine, onions, green tea, apples and berries.
Quercetin is thought to contribute to anticancer effects through several mechanisms:
-Antioxidant Activity:
-Induction of Apoptosis:modify Bax:Bcl-2 ratio
-Anti-inflammatory Effects:
-Cell Cycle Arrest:
-Inhibition of Angiogenesis and Metastasis: (VEGF)

Cellular Pathways:
-PI3K/Akt/mTOR Pathway: central to cell proliferation, survival, and metabolism.
-MAPK/ERK Pathway: influencing cell proliferation, differentiation, and apoptosis.
-NF-κB Pathway: downregulate NF-κB
-JAK/STAT Pathway: interfere with the activation of STAT3
-Apoptotic Pathways: intrinsic (mitochondrial) and extrinsic (death receptor-mediated) pathways

Quercetin has been used at doses around 500–1000 mg per day
Quercetin’s bioavailability from foods or standard supplements can be low.
EMIQ bioavialability is about 3X that of Quercetin (Example Natural Factors brand)
Isoquercitrin is high bioaviability and efficiacy but at this time does not seem available as supplement.

-Note half-life 11 to 28 hours.
BioAv low 1-10%, poor water-solubility, consuming with fat may improve bioavialability. also piperine or VitC.
Pathways:
- induce ROS production in cancer cells (higher dose). Typicallys Lowers ROS in normal cells(unless it is high dose?)or depends on Redox status?. "quercetin paradox"
- ROS↑ related: MMP↓(ΔΨm), ER Stress↑, UPR↑, GRP78↑, Ca+2↑, Cyt‑c↑, Caspases↑, DNA damage↑, cl-PARP↑, HSP↓, Prx,
- Confusing info about Lowering AntiOxidant defense in Cancer Cells: NRF2↓(some contrary), TrxR↓**, SOD↓(contrary), GSH↓ Catalase↓(contrary), HO1↓(some contrary), GPx↓(some contrary)
- Raises AntiOxidant defense in Normal Cells: ROS↓, NRF2↑, SOD↑, GSH↑, Catalase↑,
- lowers Inflammation : NF-kB↓, COX2↓, p38↓, Pro-Inflammatory Cytokines : NLRP3↓, IL-1β↓, TNF-α↓, IL-6↓, IL-8↓
- inhibit Growth/Metastases : TumMeta↓, TumCG↓, EMT↓, MMPs↓, MMP2↓, MMP9↓, TIMP2, IGF-1↓, uPA↓, VEGF↓, ROCK1↓, FAK↓, NF-κB↓, CXCR4↓, SDF1↓, TGF-β↓, α-SMA↓, ERK↓
- reactivate genes thereby inhibiting cancer cell growth : HDAC↓, DNMTs↓, EZH2↓, P53↑, HSP↓, Sp proteins↓, TET↑
- cause Cell cycle arrest : TumCCA↑, cyclin D1↓, cyclin E↓, CDK2↓, CDK4↓, CDK6↓,
- inhibits Migration/Invasion : TumCMig↓, TumCI↓, TNF-α↓, FAK↓, ERK↓, EMT↓, TOP1↓, TET1,
- inhibits glycolysis and ATP depletion : HIF-1α↓, PKM2↓, cMyc↓, GLUT1↓, LDH↓, LDHA↓, HK2↓, PFKs↓, PDKs↓, ECAR↓, OXPHOS↓, GRP78↑, GlucoseCon↓
- inhibits angiogenesis↓ : VEGF↓, HIF-1α↓, Notch↓, FGF↓, PDGF↓, EGFR↓,
- some indication of inhibiting Cancer Stem Cells : CSC↓, CK2↓, Hh↓, CD24↓, β-catenin↓, Notch2↓,
- Others: PI3K↓, AKT↓, JAK↓, STAT↓, Wnt↓, β-catenin↓, AMPK, α↓, ERK↓, JNK, - SREBP (related to cholesterol).
- Synergies: chemo-sensitization, chemoProtective, RadioSensitizer, RadioProtective, Others(review target notes), Neuroprotective, Cognitive, Renoprotection, Hepatoprotective, CardioProtective,

- Selectivity: Cancer Cells vs Normal Cells

Rank Pathway / Axis Cancer Cells Normal Cells Label Primary Interpretation Notes
1 Reactive oxygen species (ROS) ↑ ROS (dose-, metal-, context-dependent) ↓ ROS Conditional Driver Biphasic redox modulation Quercetin exhibits pro-oxidant behavior in cancer cells while protecting normal cells
2 Mitochondrial integrity / intrinsic apoptosis ↓ ΔΨm; ↑ caspase activation ↔ preserved Driver Execution of intrinsic apoptosis Mitochondrial dysfunction is a central apoptosis route in cancer cells
3 PI3K → AKT → mTOR axis ↓ AKT / ↓ mTOR ↔ adaptive suppression Driver Growth and survival inhibition AKT/mTOR suppression is a consistently reported upstream effect in cancer models
4 NF-κB signaling ↓ NF-κB activation ↓ inflammatory NF-κB tone Secondary Reduced survival and inflammatory transcription NF-κB inhibition contributes to chemosensitization and apoptosis susceptibility
5 MAPK signaling (JNK / p38) ↑ JNK / ↑ p38 ↔ minimal Secondary Stress-mediated apoptosis signaling MAPK activation supports apoptosis downstream of redox stress
6 Cell cycle regulation ↑ G1/S or G2/M arrest ↔ largely spared Phenotypic Cytostatic growth control Cell-cycle arrest reflects disruption of growth signaling
7 HIF-1α hypoxia signaling ↓ HIF-1α ↔ minimal Secondary Reduced hypoxia tolerance Quercetin interferes with hypoxia-driven transcriptional programs
8 NRF2 antioxidant response ↑ NRF2 (adaptive, context-dependent) ↑ NRF2 (protective) Adaptive Stress compensation NRF2 induction reflects redox buffering rather than primary cytotoxicity


Hif1a, HIF1α/HIF1a: Click to Expand ⟱
Source:
Type:
Hypoxia-Inducible-Factor 1A (HIF1A gene, HIF1α, HIF-1α protein product)
-Dominantly expressed under hypoxia(low oxygen levels) in solid tumor cells
-HIF1A induces the expression of vascular endothelial growth factor (VEGF)
-High HIF-1α expression is associated with Poor prognosis
-Low HIF-1α expression is associated with Better prognosis

-Functionally, HIF-1α is reported to regulate glycolysis, whilst HIF-2α regulates genes associated with lipoprotein metabolism.
-Cancer cells produce HIF in response to hypoxia in order to generate more VEGF that promote angiogenesis

Key mediators of aerobic glycolysis regulated by HIF-1α.
-GLUT-1 → regulation of the flux of glucose into cells.
-HK2 → catalysis of the first step of glucose metabolism.
-PKM2 → regulation of rate-limiting step of glycolysis.
-Phosphorylation of PDH complex by PDK → blockage of OXPHOS and promotion of aerobic glycolysis.
-LDH (LDHA): Rapid ATP production, conversion of pyruvate to lactate;

HIF-1α Inhibitors:
-Curcumin: disruption of signaling pathways that stabilize HIF-1α (ie downregulate).
-Resveratrol: downregulate HIF-1α protein accumulation under hypoxic conditions.
-EGCG: modulation of upstream signaling pathways, leading to decreased HIF-1α activity.
-Emodin: reduce HIF-1α expression. (under hypoxia).
-Apigenin: inhibit HIF-1α accumulation.


Scientific Papers found: Click to Expand⟱
910- QC,    The Anti-Cancer Effect of Quercetin: Molecular Implications in Cancer Metabolism
tumCV↓, Apoptosis↑, PI3k/Akt/mTOR↓, Wnt/(β-catenin)↓, MAPK↝, ERK↝, TumCCA↑, H2O2↑, ROS↑, TumAuto↑, MMPs↓, P53↑, Casp3↑, Hif1a↓, cFLIP↓, IL6↓, IL10↓, lactateProd↓, Glycolysis↓, PKM2↓, GLUT1↓, COX2/PTGS2↓, VEGF↓, OCR↓, ECAR↓, STAT3↓, MMP2↓, MMP9:TIMP1↓, mTOR↓,
2303- QC,  doxoR,    Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells
- in-vitro, BC, 4T1 - in-vivo, NA, NA
cardioP↑, hepatoP↑, TumCG↓, OS↑, ChemoSen↑, chemoP↑, Hif1a↓, *Hif1a↑, selectivity↑, TumVol↓, OS↑,
2300- QC,    Flavonoids Targeting HIF-1: Implications on Cancer Metabolism
- Review, Var, NA
AntiTum↑, Hif1a↓, *Hif1a↑, Glycolysis↓, HK2↓, PDK3↓, PFKP?,
76- QC,    Multifaceted preventive effects of single agent quercetin on a human prostate adenocarcinoma cell line (PC-3): implications for nutritional transcriptomics and multi-target therapy
- in-vitro, Pca, PC3
aSmase↝, Diablo↑, Fas↓, Hsc70↓, Hif1a↓, Mcl-1↓, HSP90↓, FLT4↓, EphB4↓, DNA-PK↓, PARP1↓, ATM↓, XIAP↝, PLC↓, GnT-V↝, heparanase↝, NM23↑, CSR1↑, SPP1↓, DNMT1↓, HDAC4↓, CXCR4↓, β-catenin/ZEB1↓, FBXW7↝, AMACR↓, cycD1/CCND1↓, IGF-1R↓, IMPDH1↓, IMPDH2↓, HEC1↓, NHE1↓, NOS2↓,
3353- QC,    Quercetin triggers cell apoptosis-associated ROS-mediated cell death and induces S and G2/M-phase cell cycle arrest in KON oral cancer cells
- in-vitro, Oral, KON - in-vitro, Nor, MRC-5
tumCV↓, selectivity↑, TumCCA↑, TumCMig↓, TumCI↓, Apoptosis↑, TumMeta↓, Bcl-2↓, BAX↑, TIMP1↑, MMP2↓, MMP9↓, *Inflam↓, *neuroP↑, *cardioP↑, p38↓, MAPK↓, Twist↓, P21↓, cycD1/CCND1↓, Casp3↑, Casp9↑, p‑Akt↓, p‑ERK↓, CD44↓, CD24↓, ChemoSen↑, MMP↓, Cyt‑c↑, AIF↑, ROS↑, Ca+2↑, Hif1a↓, VEGF↓,
3368- QC,    The potential anti-cancer effects of quercetin on blood, prostate and lung cancers: An update
- Review, Var, NA
*Inflam↓, *antiOx↑, *AntiCan↑, Casp3↓, p‑Akt↓, p‑mTOR↓, p‑ERK↓, β-catenin/ZEB1↓, Hif1a↓, AntiAg↓, VEGFR2/KDR/Flk1↓, EMT↓, EGFR↓, MMP2↓, MMP↓, TumMeta↓, MMPs↓, Akt↓, Snail↓, N-cadherin↓, Vim↓, E-cadherin↑, STAT3↓, TGF-β↓, ROS↓, P53↑, BAX↑, PKCδ↓, PI3K↓, COX2/PTGS2↓, cFLIP↓, cycD1/CCND1↓, cMyc↓, IL6↓, IL10↓, Cyt‑c↑, TumCCA↑, DNMTs↓, HDAC↓, ac‑H3↑, ac‑H4↑, Diablo↑, Casp3↑, Casp9↑, PARP1↑, eff↑, PTEN↑, VEGF↓, NO↓, iNOS↓, ChemoSen↑, eff↑, eff↑, eff↑, uPA↓, CXCR4↓, CXCL12↓, CLDN2↓, CDK6↓, MMP9↓, TSP-1↑, Ki-67↓, PCNA↓, ROS↑, ER Stress↑,

Showing Research Papers: 1 to 6 of 6

* indicates research on normal cells as opposed to diseased cells
Total Research Paper Matches: 6

Pathway results for Effect on Cancer / Diseased Cells:


Redox & Oxidative Stress(tgid=1)

H2O2↑, 1,   ROS↓, 1,   ROS↑, 3,  

Mitochondria & Bioenergetics(tgid=3)

AIF↑, 1,   MMP↓, 2,   OCR↓, 1,   XIAP↝, 1,  

Core Metabolism/Glycolysis(tgid=4)

AMACR↓, 1,   cMyc↓, 1,   ECAR↓, 1,   Glycolysis↓, 2,   HK2↓, 1,   lactateProd↓, 1,   PDK3↓, 1,   PFKP?, 1,   PI3k/Akt/mTOR↓, 1,   PKM2↓, 1,  

Cell Death(tgid=5)

Akt↓, 1,   p‑Akt↓, 2,   Apoptosis↑, 2,   aSmase↝, 1,   BAX↑, 2,   Bcl-2↓, 1,   Casp3↓, 1,   Casp3↑, 3,   Casp9↑, 2,   cFLIP↓, 2,   CSR1↑, 1,   Cyt‑c↑, 2,   Diablo↑, 2,   Fas↓, 1,   iNOS↓, 1,   MAPK↓, 1,   MAPK↝, 1,   Mcl-1↓, 1,   p38↓, 1,  

Transcription & Epigenetics(tgid=7)

ac‑H3↑, 1,   ac‑H4↑, 1,   SPP1↓, 1,   tumCV↓, 2,  

Protein Folding & ER Stress(tgid=8)

ER Stress↑, 1,   Hsc70↓, 1,   HSP90↓, 1,  

Autophagy & Lysosomes(tgid=9)

TumAuto↑, 1,  

DNA Damage & Repair(tgid=10)

ATM↓, 1,   DNA-PK↓, 1,   DNMT1↓, 1,   DNMTs↓, 1,   P53↑, 2,   PARP1↓, 1,   PARP1↑, 1,   PCNA↓, 1,  

Cell Cycle & Senescence(tgid=11)

cycD1/CCND1↓, 3,   P21↓, 1,   TumCCA↑, 3,  

Proliferation, Differentiation & Cell State(tgid=12)

CD24↓, 1,   CD44↓, 1,   EMT↓, 1,   ERK↝, 1,   p‑ERK↓, 2,   FBXW7↝, 1,   HDAC↓, 1,   HDAC4↓, 1,   IGF-1R↓, 1,   mTOR↓, 1,   p‑mTOR↓, 1,   PI3K↓, 1,   PTEN↑, 1,   STAT3↓, 2,   TumCG↓, 1,   Wnt/(β-catenin)↓, 1,  

Migration(tgid=13)

AntiAg↓, 1,   Ca+2↑, 1,   CLDN2↓, 1,   CXCL12↓, 1,   E-cadherin↑, 1,   EphB4↓, 1,   GnT-V↝, 1,   heparanase↝, 1,   Ki-67↓, 1,   MMP2↓, 3,   MMP9↓, 2,   MMP9:TIMP1↓, 1,   MMPs↓, 2,   N-cadherin↓, 1,   NM23↑, 1,   PKCδ↓, 1,   Snail↓, 1,   TGF-β↓, 1,   TIMP1↑, 1,   TSP-1↑, 1,   TumCI↓, 1,   TumCMig↓, 1,   TumMeta↓, 2,   Twist↓, 1,   uPA↓, 1,   Vim↓, 1,   β-catenin/ZEB1↓, 2,  

Angiogenesis & Vasculature(tgid=14)

EGFR↓, 1,   FLT4↓, 1,   Hif1a↓, 6,   NO↓, 1,   VEGF↓, 3,   VEGFR2/KDR/Flk1↓, 1,  

Barriers & Transport(tgid=15)

GLUT1↓, 1,   NHE1↓, 1,  

Immune & Inflammatory Signaling(tgid=16)

COX2/PTGS2↓, 2,   CXCR4↓, 2,   IL10↓, 2,   IL6↓, 2,  

Cellular Microenvironment(tgid=17)

PLC↓, 1,  

Hormonal & Nuclear Receptors(tgid=20)

CDK6↓, 1,  

Drug Metabolism & Resistance(tgid=21)

ChemoSen↑, 3,   eff↑, 4,   selectivity↑, 2,  

Clinical Biomarkers(tgid=22)

EGFR↓, 1,   HEC1↓, 1,   IL6↓, 2,   Ki-67↓, 1,   NOS2↓, 1,  

Functional Outcomes(tgid=23)

AntiTum↑, 1,   cardioP↑, 1,   chemoP↑, 1,   hepatoP↑, 1,   IMPDH1↓, 1,   IMPDH2↓, 1,   OS↑, 2,   TumVol↓, 1,  
Total Targets: 128

Pathway results for Effect on Normal Cells:


Redox & Oxidative Stress(tgid=1)

antiOx↑, 1,  

Angiogenesis & Vasculature(tgid=14)

Hif1a↑, 2,  

Immune & Inflammatory Signaling(tgid=16)

Inflam↓, 2,  

Functional Outcomes(tgid=23)

AntiCan↑, 1,   cardioP↑, 1,   neuroP↑, 1,  
Total Targets: 6

Scientific Paper Hit Count for: Hif1a, HIF1α/HIF1a
6 Quercetin
1 doxorubicin
Query results interpretion may depend on "conditions" listed in the research papers.
Such Conditions may include : 
  -low or high Dose
  -format for product, such as nano of lipid formations
  -different cell line effects
  -synergies with other products 
  -if effect was for normal or cancerous cells
Filter Conditions: Pro/AntiFlg:%  IllCat:%  CanType:%  Cells:%  prod#:140  Target#:143  State#:%  Dir#:1
wNotes=0 sortOrder:rid,rpid

 

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